Enhanced metabolic flux of methylerythritol phosphate (MEP) pathway by overexpression of Ginkgo biloba 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Reductase 1 (GbHDR1) gene in poplar

Abstract Terpenoids are of great interests in a broad range health-beneficial biological activities and various industrial applications. In plants, terpenoids synthesized by two distinct pathways, methylerythritol phosphate (MEP) mevalonate pathways separate location. MEP pathway supplies isoprene precursors isopentenyl diphosphate (IPP) its isomer dimethylallyl (DMAPP) terpenoid biosynthesis plant plastids. The has been an engineering target to increase the metabolic flux towards higher production plants. 1-Hydroxy-2-methyl-2-( E )-butenyl-4-diphosphate reductase (HDR) is terminal step regulate encoded three paralogous genes Ginkgo biloba. this study, we assessed effect overexpression GbHDR1 on metabolism poplar Overexpression plants accelerated growth delayed winter-bud formation. Transcript levels gibberellin, chlorophylls, carotenoid biosynthetic GbHDR1- overexpressing ( ox) poplars were up-regulated, suggesting enhancement. Moreover, enhanced contents chlorophylls carotenoids leaves ox resulted photosynthetic rate as consequence. Therefore, expect will be desirable point for enhancing